Characterization of Gypsy Moth ( Lymantria dispar ) Nuclear Polyhedrosis Virus
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چکیده
منابع مشابه
Characterization of Gypsy Moth (Lymantria dispar) Nuclear Polyhedrosis Virus.
Characterization of the proteins and nucleic acid of the gypsy moth nuclear polyhedrosis virus isolated in Ithaca, N.Y. (LdNPV-IT) is presented. A total of 29 viral structural proteins were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis when the virus was isolated in the absence of alkaline protease activity. Fourteen surface envelope viral proteins were identified by l...
متن کاملA field release of genetically engineered gypsy moth (Lymantria dispar L.) nuclear polyhedrosis virus (LdNPV).
The gypsy moth (Lymantria dispar L.) nuclear polyhedrosis virus was genetically engineered for nonpersistence by removal of the gene coding for polyhedrin production and stabilized using a coocclusion process. A beta-galactosidase marker gene was inserted into the genetically engineered virus (LdGEV) so that infected larvae could be tested for its presence using a colorimetric assay. In 1993, L...
متن کاملInteractions between a Nosema sp. (Microspora: nosematidae) and nuclear polyhedrosis virus infecting the gypsy moth, Lymantria dispar (Lepidoptera: lymantriidae).
Simultaneous and sequential per os inoculations of gypsy moth larvae with the Lymantria dispar nuclear polyhedrosis virus (LdNPV) and a Nosema sp. from Portugal demonstrated that the interaction of two pathogens during coinfection was variable, ranging from synergistic to antagonistic. Susceptibility of gypsy moth larvae to viral infection was unaffected by simultaneous and subsequent microspor...
متن کاملCharacterization of the replication cycle of the Lymantria dispar nuclear polyhedrosis virus.
The life cycle of the Lymantria dispar nuclear polyhedrosis virus (LdMNPV) was characterized through analysis of budded virus (BV) release, the temporal formation of polyhedra, the temporal transcription pattern of representative early, late, and hyper-expressed late genes, and the onset of DNA replication in the Ld652Y cell line. Transcripts from the LdMNPV immediate early gene G22 were detect...
متن کاملIdentification and characterization of an early gene in the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus.
The Lymantria dispar multinucleocapsid nuclear polyhedrosis virus (LdMNPV) gene encoding G22 was cloned and sequenced. The G22 gene codes for a 191 amino acid protein with a predicted M(r) of 22,000. Expression of G22 in a rabbit reticulocyte system generated a protein with an M(r) of 24,000, in close agreement with the molecular mass predicted from the nucleotide sequence. G22 is not significa...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1983
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.46.2.297-303.1983